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验证数据展示

  • WB analysis using 68495-1-Ig

    WB analysis using 68495-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HepG2 using 68495-1-Ig

    WB analysis of HepG2 using 68495-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TJAP1 Monoclonal antibody

TJAP1 Monoclonal Antibody for WB, ELISA
Cat No. 68495-1-Ig

产品说明书

CloneNo. 1H1F10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse , Rat

应用

WB, ELISA

别名

DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, HSC-T6 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68495-1-Ig targets TJAP1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse , Rat
免疫原 TJAP1 fusion protein Ag11807 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tight junction associated protein 1 (peripheral)
别名 DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4
计算分子量 547 aa, 61 kDa
观测分子量 86-95 kDa
GenBank蛋白编号BC064401
基因名称 TJAP1
Gene ID (NCBI) 93643
RRIDAB_3085206
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ5JTD0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

实验方案

Product Specific Protocols
WB protocol for TJAP1 antibody 68495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols