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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30755-1-AP (same clone as 30755-1-PBS)

    WB analysis using 30755-1-AP (same clone as 30755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30755-1-AP (TIMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30755-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 30755-1-AP (same clone as 30755-1-PBS)

    IF Staining of HepG2 using 30755-1-AP (same clone as 30755-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TIMP1 antibody (30755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30755-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30755-1-AP (same clone as 30755-1-PBS)

    IF Staining of HeLa using 30755-1-AP (same clone as 30755-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (30755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30755-1-PBS in a different storage buffer formulation.

  • FC experiment of HT-29 using 30755-1-AP (same clone as 30755-1-PBS)

    FC experiment of HT-29 using 30755-1-AP (same clone as 30755-1-PBS)

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human TIMP1 (30755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 30755-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 30755-1-AP (same clone as 30755-1-PBS)

    IHC staining of rat testis using 30755-1-AP (same clone as 30755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30755-1-AP (TIMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30755-1-PBS in a different storage buffer formulation.

TIMP1 Polyclonal antibody, PBS Only

TIMP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 30755-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30755-1-PBS targets TIMP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 TIMP1 fusion protein Eg0638 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TIMP metallopeptidase inhibitor 1
别名 TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity
计算分子量 23 kDa
观测分子量 23-28 kDa
GenBank蛋白编号BC000866
基因名称 TIMP1
Gene ID (NCBI) 7076
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01033
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.