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验证数据展示

  • WB analysis using 30500-1-AP (same clone as 30500-1-PBS)

    WB analysis using 30500-1-AP (same clone as 30500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30500-1-AP (TAPBP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30500-1-AP (same clone as 30500-1-PBS)

    IF Staining of HeLa using 30500-1-AP (same clone as 30500-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TAPBP antibody (30500-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

TAPBP Polyclonal antibody, PBS Only

TAPBP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30500-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30500-1-PBS targets TAPBP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30885

Product name: Recombinant human TAPBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-120 aa of NM_003190

Sequence: GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAP binding protein (tapasin)
别名
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号NM_003190
基因名称 TAPBP
Gene ID (NCBI) 6892
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15533
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAPBP, also known as tapasin, is a V-C1 (variable-constant) immunoglobulin superfamily (IgSF) molecule. It is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (PMID: 11920573).

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