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TAPBP Polyclonal antibody

TAPBP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30500-1-AP

Print datasheet

Synonyms

NGS 17, NGS17, TAP associated protein, TAP binding protein, TAP binding protein (tapasin), TAPA, tapasin, TAPBP, TPN, TPSN

验证数据展示

  • WB analysis using 30500-1-AP

    WB analysis using 30500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30500-1-AP (TAPBP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 30500-1-AP

    IHC staining of mouse colon using 30500-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 30500-1-AP

    IHC staining of mouse colon using 30500-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 30500-1-AP

    IF Staining of HeLa using 30500-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TAPBP antibody (30500-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells, THP-1 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30500-1-AP targets TAPBP in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen TAPBP fusion protein Ag30885 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAP binding protein (tapasin)
Synonyms NGS 17, NGS17, TAP associated protein, TAP binding protein, TAP binding protein (tapasin), TAPA, tapasin, TAPBP, TPN, TPSN
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberNM_003190
Gene Symbol TAPBP
Gene ID (NCBI) 6892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAPBP, also known as tapasin, is a V-C1 (variable-constant) immunoglobulin superfamily (IgSF) molecule. It is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (PMID: 11920573).

实验方案

Product Specific Protocols
WB protocol for TAPBP antibody 30500-1-APDownload protocol
IHC protocol for TAPBP antibody 30500-1-APDownload protocol
IF protocol for TAPBP antibody 30500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols