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验证数据展示

  • WB analysis using 28713-1-AP (same clone as 28713-1-PBS)

    WB analysis using 28713-1-AP (same clone as 28713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28713-1-AP (TAF9B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 28713-1-AP (same clone as 28713-1-PBS)

    IP experiment of HeLa using 28713-1-AP (same clone as 28713-1-PBS)

    IP result of anti-TAF9B (IP:28713-1-AP, 4ug; Detection:28713-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 28713-1-AP (same clone as 28713-1-PBS)

    IHC staining of mouse testis using 28713-1-AP (same clone as 28713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28713-1-AP (TAF9B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

TAF9B Polyclonal antibody, PBS Only

TAF9B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 28713-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28713-1-PBS targets TAF9B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29633

Product name: Recombinant human TAF9B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 133-200 aa of BC010350

Sequence: IKKGPNQGRLVPRLSVGAVSSKPTTPTIATPQTVSVPNKVATPMSVTSQRFTVQIPPSQSTPVKPVPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
别名
计算分子量 28 kDa
观测分子量30 kDa
GenBank蛋白编号BC010350
基因名称 TAF9B
Gene ID (NCBI) 51616
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBM6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7, transcription initiation factor TFIID subunit 9-like, transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes, may have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. The calculated MW of TAF9B is 28 kDa, 28713-1-AP can detect a band around 30 kDa.

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