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验证数据展示

  • WB analysis using 33001-1-AP

    WB analysis using 33001-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33001-1-AP (Surfactant protein D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 33001-1-AP

    IHC staining of mouse lung using 33001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33001-1-AP (Surfactant protein D antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 33001-1-AP

    IHC staining of rat lung using 33001-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 33001-1-AP (Surfactant protein D antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Surfactant protein D Polyclonal antibody

Surfactant protein D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33001-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33001-1-AP targets Surfactant protein D in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2924

Product name: Recombinant Mouse Surfactant protein D protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-374 aa of NM_009160.2

Sequence: AEMKSLSQRSVPNTCTLVMCSPTENGLPGRDGRDGREGPRGEKGDPGLPGPMGLSGLQGPTGPVGPKGENGSAGEPGPKGERGLSGPPGLPGIPGPAGKEGPSGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSTGAKGSTGPKGERGAPGVQGAPGNAGAAGPAGPAGPQGAPGSRGPPGLKGDRGVPGDRGIKGESGLPDSAALRQQMEALKGKLQRLEVAFSHYQKAALFPDGRSVGDKIFRTADSEKPFEDAQEMCKQAGGQLASPRSATENAAIQQLITAHNKAAFLSMTDVGTEGKFTYPTGEPLVYSNWAPGEPNNNGGAENCVEIFTNGQWNDKACGEQRLVICEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 surfactant associated protein D
别名 Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd
计算分子量38 kDa
观测分子量40 kDa
GenBank蛋白编号NM_009160.2
基因名称 Sftpd
Gene ID (NCBI) 20390
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP50404
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Surfactant protein D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.

实验方案

Product Specific Protocols
WB protocol for Surfactant protein D antibody 33001-1-APDownload protocol
IHC protocol for Surfactant protein D antibody 33001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols