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验证数据展示

  • IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

    IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

    IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13915-1-AP (same clone as 13915-1-PBS)

    IP experiment of mouse testis using 13915-1-AP (same clone as 13915-1-PBS)

    IP result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • WB analysis using 13915-1-AP (same clone as 13915-1-PBS)

    WB analysis using 13915-1-AP (same clone as 13915-1-PBS)

    DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 13915-1-AP (SPAG8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 13915-1-AP (same clone as 13915-1-PBS)

    IF Staining of PC-3 using 13915-1-AP (same clone as 13915-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SPAG8 antibody (13915-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

SPAG8 Polyclonal antibody, PBS Only

SPAG8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13915-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

HSD-1, HSD 1, CT142, CILD28, BS84

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13915-1-PBS targets SPAG8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4910

Product name: Recombinant human SPAG8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC019247

Sequence: METNGSTEGSRSRSRSLDIQPSSEGLGPTSEPFPSSDDSPRSALAAATAAAAAAASAAAATAAFTTAKAAALSTKTPAPCSEFMEPSSDPSLLGEPCAGPGFTHNIAHGSLGFEPVYVSCIAQDTCTTTDHSSNPGPVPGSSSGPVLGSSSGAGHGSGSGSGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFRNLVADRVLNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKPPDIKGKLMVCYETLPRGQCLLYNWEEERATNHLDQVPSMQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 8
别名 HSD-1, HSD 1, CT142, CILD28, BS84
计算分子量 45 kDa, 54 kDa
观测分子量 56-70 kDa
GenBank蛋白编号BC019247
基因名称 SPAG8
Gene ID (NCBI) 26206
RRIDAB_2194804
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99932
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.

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