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验证数据展示

  • WB analysis of HepG2 using 17594-1-AP (same clone as 17594-1-PBS)

    WB analysis of HepG2 using 17594-1-AP (same clone as 17594-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17594-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 17594-1-AP (same clone as 17594-1-PBS)

    IHC staining of human pancreas using 17594-1-AP (same clone as 17594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17594-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 17594-1-AP (same clone as 17594-1-PBS)

    IHC staining of human pancreas using 17594-1-AP (same clone as 17594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17594-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 17594-1-AP (same clone as 17594-1-PBS)

    IF Staining of HepG2 using 17594-1-AP (same clone as 17594-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17594-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SMYD4 Polyclonal antibody, PBS Only

SMYD4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17594-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ZMYND21, SMYD 4, SET and MYND domain-containing protein 4, EC:2.1.1.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17594-1-PBS targets SMYD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag11552

Product name: Recombinant human SMYD4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 201-578 aa of BC035077

Sequence: KDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLVQEDAFVSVLNPGELPPPHHGLDSKWDTRVTNGDLYCHRCLKHTLATVPCDGCSYAKYCSQECLQQVWELYHRTECPLGGLLLTLGVFCHIALRLTLLVGFEDVRKIITKLCDKISNKDICLPESNNQVKTLNYGLGESEKNGNIVETPIPGCDINGKYENNYNAVFNLLPHTENHSPEHKFLCALCVSALCRQLEAASLQAIPTERIVNSSQLKAAVTPELCPDVTIWGVAMLRHMLQLQCNAQAMTTIQHTGPKGSIVTDSRQVRLATGIFPVISLLNHSCSPNTSVSFISTVATIRASQRIRKGQEILHCYGPHKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET and MYND domain containing 4
别名 ZMYND21, SMYD 4, SET and MYND domain-containing protein 4, EC:2.1.1.-
计算分子量 804 aa, 89 kDa
观测分子量 89 kDa
GenBank蛋白编号BC035077
基因名称 SMYD4
Gene ID (NCBI) 114826
RRIDAB_10694429
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYR2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMYD4, also named as SET and MYND domain-containing protein 4, is a 804 amino acid protein, which contains 1 MYND-type zinc finger and 1 SET domain. SMYD4 belongs to the class V-like SAM-binding methyltransferase superfamily. SMYD4, as a potential tumor suppressor, plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of Pdgfr-alpha, and could be a novel target for improving treatment of breast cancer. SMYD4 is a muscle-specific transcriptional modulator involved in development.

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