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验证数据展示

  • WB analysis of HeLa using 16397-1-AP (same clone as 16397-1-PBS)

    WB analysis of HeLa using 16397-1-AP (same clone as 16397-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 16397-1-AP (same clone as 16397-1-PBS)

    IHC staining of human prostate cancer using 16397-1-AP (same clone as 16397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16397-1-AP (SMAD9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD9 antibody included
  • IHC staining of mouse heart using 16397-1-AP (same clone as 16397-1-PBS)

    IHC staining of mouse heart using 16397-1-AP (same clone as 16397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16397-1-AP (SMAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD9 antibody included
  • IHC staining of rat heart using 16397-1-AP (same clone as 16397-1-PBS)

    IHC staining of rat heart using 16397-1-AP (same clone as 16397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 16397-1-AP (SMAD9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD9 antibody included
  • IHC staining of human prostate cancer using 16397-1-AP (same clone as 16397-1-PBS)

    IHC staining of human prostate cancer using 16397-1-AP (same clone as 16397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16397-1-AP (SMAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD9 antibody included
  • IHC staining of human urothelial carcinoma using 16397-1-AP (same clone as 16397-1-PBS)

    IHC staining of human urothelial carcinoma using 16397-1-AP (same clone as 16397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16397-1-AP (SMAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD9 antibody included

SMAD9 Polyclonal antibody, PBS Only

SMAD9 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16397-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

MAD homolog 9, MADH6, MADH9, Mothers against DPP homolog 9, SMAD 9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMAD9 Ready-To-Use IHC Kit. SMAD9 primary antibody included.

产品信息

16397-1-PBS targets SMAD9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9490

Product name: Recombinant human SMAD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-352 aa of BC011559

Sequence: MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLPPVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQPPCSALPPSPSHAFSQSPCTASYPHSPGSPSEPESPYQHSDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMAD family member 9
别名 MAD homolog 9, MADH6, MADH9, Mothers against DPP homolog 9, SMAD 9
计算分子量 430 aa, 49 kDa
观测分子量 49-55 kDa
GenBank蛋白编号BC011559
基因名称 SMAD9
Gene ID (NCBI) 4093
RRIDAB_2270847
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15198
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD9 (also known as MADH9 or MAD homolog 9) is a key signaling molecule in the transforming growth factor-β (TGF-β) superfamily signaling pathway and belongs to the SMAD1/5/9 subgroup of receptor-regulated SMADs (R-SMADs). It is primarily activated and phosphorylated by BMP (bone morphogenetic protein) type I receptors. Once activated, SMAD9 forms a complex with the common SMAD4 (Co-SMAD), translocates into the nucleus, and acts as a transcription factor to regulate the expression of specific target genes. This process is involved in modulating a variety of critical biological processes, including cell proliferation, differentiation, apoptosis, and embryonic development. Mutations or abnormal expression of SMAD9 are associated with several diseases, particularly in pulmonary arterial hypertension, certain cancers, and vascular diseases, where it plays a significant role.

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