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验证数据展示

  • WB analysis using 30130-1-AP (same clone as 30130-1-PBS)

    WB analysis using 30130-1-AP (same clone as 30130-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30130-1-AP (SMAD3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 30130-1-AP (same clone as 30130-1-PBS)

    IHC staining of mouse stomach using 30130-1-AP (same clone as 30130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included
  • IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

    IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included
  • IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

    IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included
  • IHC staining of rat stomach using 30130-1-AP (same clone as 30130-1-PBS)

    IHC staining of rat stomach using 30130-1-AP (same clone as 30130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included

SMAD3 Polyclonal antibody, PBS Only

SMAD3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30130-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

hMAD 3, hMAD-3, hSMAD3, HSPC193, HsT17436

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMAD3 Ready-To-Use IHC Kit. SMAD3 primary antibody included.

产品信息

30130-1-PBS targets SMAD3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32595

Product name: Recombinant human SMAD3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 145-260 aa of NM_005902

Sequence: EIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMAD family member 3
别名 hMAD 3, hMAD-3, hSMAD3, HSPC193, HsT17436
计算分子量 48 kDa
观测分子量48-55 kDa
GenBank蛋白编号NM_005902
基因名称 SMAD3
Gene ID (NCBI) 4088
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP84022
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

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