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验证数据展示

  • WB analysis using 12441-1-AP (same clone as 12441-1-PBS)

    WB analysis using 12441-1-AP (same clone as 12441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12441-1-AP (same clone as 12441-1-PBS)

    IHC staining of human prostate cancer using 12441-1-AP (same clone as 12441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12441-1-AP (SHPK antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

SHPK Polyclonal antibody, PBS Only

SHPK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12441-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12441-1-PBS targets SHPK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3150

Product name: Recombinant human SHPK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 131-478 aa of BC020543

Sequence: RCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNISTSVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTVYSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQEWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sedoheptulokinase
别名
计算分子量 478 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC020543
基因名称 SHPK
Gene ID (NCBI) 23729
RRIDAB_2188566
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHJ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

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