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验证数据展示

  • WB analysis using 16977-1-AP (same clone as 16977-1-PBS)

    WB analysis using 16977-1-AP (same clone as 16977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16977-1-AP (SGOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16977-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16977-1-AP (same clone as 16977-1-PBS)

    IF Staining of HeLa using 16977-1-AP (same clone as 16977-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SGOL1 antibody (16977-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16977-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SGOL1 Polyclonal antibody, PBS Only

SGOL1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16977-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

hSgo1, NY BR 85, Serologically defined breast cancer antigen NY-BR-85, SGO, SGO1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16977-1-PBS targets SGOL1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9700

Product name: Recombinant human SGOL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-292 aa of BC017867

Sequence: MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRRSFIAAPCQIITNTSTLLKNYQDNNKMLVLALENEKSKVKEAQDIILQLRKECYYLTCQLYALKGKLTSQQTVEPAQNQEICSSGMDPNSDDSSRNLFVKDLPQIPLEETELPGQGESFQIEATPPETQQSPHLSLKDITNVSLYPVVKIRRLSLSPKKNKASPAVALPKRRCTASVNYKEPTLASKLRRGDPFTDLCFLNSPIFKQKKDLRRSKKRALEVSPAKEAIFILYYVREFVSRFPDCRKCKLETHICLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 shugoshin-like 1 (S. pombe)
别名 hSgo1, NY BR 85, Serologically defined breast cancer antigen NY-BR-85, SGO, SGO1
计算分子量561aa,64 kDa; 292aa,34 kDa
观测分子量 64 kDa
GenBank蛋白编号BC017867
基因名称 SGOL1
Gene ID (NCBI) 151648
RRIDAB_2188437
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5FBB7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SGOL1 (Shugoshin 1), a centromeric protein, plays an important role in mitosis (PMID: 23102702).