• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 15328-1-AP (same clone as 15328-1-PBS)

    WB analysis of HEK-293 using 15328-1-AP (same clone as 15328-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

    IP experiment of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

    IP result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFRP4 antibody included
  • IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFRP4 antibody included
  • IHC staining of rat testis using 15328-1-AP (same clone as 15328-1-PBS)

    IHC staining of rat testis using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFRP4 antibody included
  • Featured Product
  • KD/KO验证

SFRP4 Polyclonal antibody, PBS Only

SFRP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15328-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy SFRP4 Ready-To-Use IHC Kit. SFRP4 primary antibody included.

产品信息

15328-1-PBS targets SFRP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 SFRP4 fusion protein Ag6909 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 secreted frizzled-related protein 4
别名
计算分子量 40 kDa
观测分子量 41-51 kDa, 55 kDa
GenBank蛋白编号BC058911
基因名称 SFRP4
Gene ID (NCBI) 6424
RRIDAB_2187099
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6FHJ7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.