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验证数据展示

  • WB analysis using 24840-1-AP (same clone as 24840-1-PBS)

    WB analysis using 24840-1-AP (same clone as 24840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24840-1-AP (same clone as 24840-1-PBS)

    IF Staining of HeLa using 24840-1-AP (same clone as 24840-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SETD7 Polyclonal antibody, PBS Only

SETD7 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24840-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

24840-1-PBS targets SETD7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18219

Product name: Recombinant human SETD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-366 aa of BC121055

Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing (lysine methyltransferase) 7
别名
计算分子量 366 aa, 41 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC121055
基因名称 SETD7
Gene ID (NCBI) 80854
RRIDAB_2737406
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WTS6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

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