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验证数据展示

  • WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

    WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

    IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SETD3 antibody included
  • IF Staining of MCF-7 using 28692-1-AP (same clone as 28692-1-PBS)

    IF Staining of MCF-7 using 28692-1-AP (same clone as 28692-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 28692-1-AP (same clone as 28692-1-PBS)

    IF Staining of SH-SY5Y using 28692-1-AP (same clone as 28692-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

SETD3 Polyclonal antibody, PBS Only

SETD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28692-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C14orf154, SET domain containing 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SETD3 Ready-To-Use IHC Kit. SETD3 primary antibody included.

产品信息

28692-1-PBS targets SETD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30017

Product name: Recombinant human SETD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of NM_032233

Sequence: MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing 3
别名 C14orf154, SET domain containing 3
计算分子量 67 kDa
观测分子量67 kDa
GenBank蛋白编号NM_032233
基因名称 SETD3
Gene ID (NCBI) 84193
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TU7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).

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