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验证数据展示

  • WB analysis using 28692-1-AP

    WB analysis using 28692-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 28692-1-AP

    IHC staining of mouse small intestine using 28692-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SETD3 antibody included

SETD3 Polyclonal antibody

SETD3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28692-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SET domain containing 3, C14orf154

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inmouse small intestine tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SETD3 Ready-To-Use IHC Kit. SETD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28692-1-AP targets SETD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SETD3 fusion protein Ag30017 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing 3
别名 SET domain containing 3, C14orf154
计算分子量 67 kDa
观测分子量67 kDa
GenBank蛋白编号NM_032233
基因名称 SETD3
Gene ID (NCBI) 84193
RRIDAB_3086077
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).

实验方案

Product Specific Protocols
WB protocol for SETD3 antibody 28692-1-APDownload protocol
IHC protocol for SETD3 antibody 28692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols