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验证数据展示

  • WB analysis using 13673-1-AP (same clone as 13673-1-PBS)

    WB analysis using 13673-1-AP (same clone as 13673-1-PBS)

    Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13673-1-AP (SEC24D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    IHC staining of human pancreas using 13673-1-AP (same clone as 13673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13673-1-AP (same clone as 13673-1-PBS)

    IF Staining of HeLa using 13673-1-AP (same clone as 13673-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13673-1-AP (SEC24D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13673-1-PBS in a different storage buffer formulation.

SEC24D Polyclonal antibody, PBS Only

SEC24D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13673-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13673-1-PBS targets SEC24D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4657

Product name: Recombinant human SEC24D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 783-1033 aa of BC035761

Sequence: TDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC24 family, member D (S. cerevisiae)
别名
计算分子量 1033 aa, 113 kDa
观测分子量 113 kDa
GenBank蛋白编号BC035761
基因名称 SEC24D
Gene ID (NCBI) 9871
RRIDAB_2877968
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94855
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

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