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0
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验证数据展示

  • WB analysis using 23006-1-AP (same clone as 23006-1-PBS)

    WB analysis using 23006-1-AP (same clone as 23006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    IP experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    IP result of anti-SCP2 (IP:23006-1-AP, 4ug; Detection:23006-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    IF Staining of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23006-1-AP (SCP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 23006-1-AP (same clone as 23006-1-PBS)

    WB analysis of HEK-293 using 23006-1-AP (same clone as 23006-1-PBS)

    WB result of SCP2 antibody (23006-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    FC experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SCP2 (23006-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SCP2 Polyclonal antibody, PBS Only

SCP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 23006-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

23006-1-PBS targets SCP2 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SCP2 fusion protein Ag19215 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterol carrier protein 2
别名
计算分子量 547 aa, 59 kDa
观测分子量 13-15 kDa
GenBank蛋白编号BC005911
基因名称 SCP2
Gene ID (NCBI) 6342
RRIDAB_2879197
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22307
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.