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验证数据展示

  • WB analysis using 25544-1-AP (same clone as 25544-1-PBS)

    WB analysis using 25544-1-AP (same clone as 25544-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    WB analysis of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    WB result of SCML2 antibody (25544-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML2 transfected HEK-293 cells. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    IP experiment of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    IP result of anti-SCML2 (IP:25544-1-AP, 4ug; Detection:25544-1-AP 1:300) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    IF Staining of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 25544-1-AP (SCML2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SCML2 Polyclonal antibody, PBS Only

SCML2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 25544-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

25544-1-PBS targets SCML2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SCML2 fusion protein Ag22271 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex comb on midleg-like 2 (Drosophila)
别名
计算分子量 700 aa, 77 kDa
观测分子量 70 kDa, 80 kDa
GenBank蛋白编号BC064617
基因名称 SCML2
Gene ID (NCBI) 10389
RRIDAB_2880127
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQR0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic (PMID: 24358021). Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a Polycomb-group protein that associates with PRC1. SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1 (PMID: 24986859).