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SAG

SAG (Smoothened Agonist) 是 Smoothened (Smo)受体激动剂 (EC50=3 nM;Kd=59 nM)。它能够激活 Hedgehog 信号通路，抵消 Cyclopamine 对 Smo 的抑制作用。

CAS号

912545-86-9

分子式

C28H28ClN3OS

主要靶点

Hedgehog/Smoothened|Smo

仅限科研使用

Cat No : CM06477

Print datasheet

Synonyms

Smoothened Agonist|Smoothened Agonist (SAG) HCl

验证数据展示

  • CAS No.: 912545-86-9

    SAG
    CAS#: 912545-86-9

产品信息

SAG (Smoothened Agonist) is a potent Smo receptor agonist and activates the Hedgehog signaling pathway.

CAS号 912545-86-9
分子式 C28H28ClN3OS
主要靶点 Hedgehog/Smoothened|Smo
主要通路 干细胞|G蛋白偶联受体
分子量 490.06
纯度 98.41%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 Smoothened Agonist|Smoothened Agonist (SAG) HCl

靶点活性

Smoothened:3 nM(EC50)

体内活性

In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. [3] In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. [4]

体外活性

SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. [1] SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. [2] SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. [3]

溶解度

DMSO:71 mg/mL (134.84 mM),warmed,Ethanol:40 mg/mL (75.97 mM),warmed

参考文献

1.Chen JK, et al. Proc Natl Acad Sci U S A. 2002, 99(22), 14071-14076.
2.Meloni AR, et al. Mol Cell Biol. 2006, 26(20), 7550-760.
3.Bragina O, et al. Neurosci Lett. 2010, 482(2), 81-85.
4.Heine VM, et al. Sci Transl Med. 2011, 3(105), 105ra104.
5.Schenke M, Schjeide B M, Püschel G P, et al. Analysis of Motor Neurons Differentiated from Human Induced Pluripotent Stem Cells for the Use in Cell-Based Botulinum Neurotoxin Activity Assays[J]. Toxins. 2020, 12(5): 276.
6.Schenke M. DOCTOR OF PHILOSOPHY (PhD)[D]. University of Veterinary Medicine Hannover. 2020

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2