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验证数据展示

  • FC experiment of C57BL/6 bone marrow cells using 98505-5-RR (same clone as 98505-5-PBS)

    FC experiment of C57BL/6 bone marrow cells using 98505-5-RR (same clone as 98505-5-PBS)

    1x10^6 C57BL/6 bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse S100a9 Rabbit RecAb (98505-5-RR, Clone:250759F10), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were then stained with FITC Plus Anti-Mouse Ly-6G/Ly-6C (Gr-1). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98505-5-PBS in a different storage buffer formulation.

  • FC experiment of C57BL/6 bone marrow cells using 98505-5-RR (same clone as 98505-5-PBS)

    FC experiment of C57BL/6 bone marrow cells using 98505-5-RR (same clone as 98505-5-PBS)

    1x10^6 C57BL/6 bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse S100a9 Rabbit RecAb (98505-5-RR, Clone:250759F10)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98505-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 98505-5-RR (same clone as 98505-5-PBS)

    IHC staining of mouse spleen using 98505-5-RR (same clone as 98505-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 98505-5-RR (S100A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98505-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 98505-5-RR (same clone as 98505-5-PBS)

    IHC staining of mouse spleen using 98505-5-RR (same clone as 98505-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 98505-5-RR (S100A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98505-5-PBS in a different storage buffer formulation.

  • Affinity of 98505-5-RR

    Affinity of 98505-5-RR

    Biolayer interferometry (BLl) kinetic assays of 98505-5-RR against Mouse S100A9 were performed. The affinity constant is 13.9 nM.

Anti-Mouse S100A9 Rabbit Recombinant Antibody, PBS Only

S100A9 Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98505-5-PBS

产品说明书

CloneNo. 250759F10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, FC (Intra)

别名

Cagb, Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, Mrp14

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98505-5-PBS targets S100A9 in IHC, FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2600

Product name: Recombinant Mouse S100a9 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 2-113 aa of NM_001281852.1

Sequence: ANKAPSQMERSITTIIDTFHQYSRKEGHPDTLSKKEFRQMVEAQLATFMKKEKRNEALINDIMEDLDTNQDNQLSFEECMMLMAKLIFACHEKLHENNPRGHGHSHGKGCGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A9 (calgranulin B)
别名 Cagb, Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, Mrp14
计算分子量13 kDa
GenBank蛋白编号NM_001281852.1
基因名称 S100a9
Gene ID (NCBI) 20202
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31725
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

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