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验证数据展示

  • WB analysis using 32681-1-AP

    WB analysis using 32681-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32681-1-AP (S100a8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 32681-1-AP

    IP experiment of mouse spleen using 32681-1-AP

    IP result of anti-S100A8 (IP:32681-1-AP, 4ug; Detection:32681-1-AP 1:3000) with mouse spleen tissue lysate 2480 ug.

  • IF Staining of NIH/3T3 using 32681-1-AP

    IF Staining of NIH/3T3 using 32681-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using S100A8 antibody (32681-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

S100A8 Polyclonal antibody

S100A8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 32681-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat spleen tissue, mouse spleen tissue
Positive IP detected inmouse spleen tissue
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32681-1-AP targets S100A8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2959

Product name: recombinant mouse S100a8 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 2-89 aa of NM_013650.2

Sequence: PSELEKALSNLIDVYHNYSNIQGNHHALYKNDFKKMVTTECPQFVQNINIENLFRELDINSDNAINFEEFLAMVIKVGVASHKDSHKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A8 (calgranulin A)
别名 Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8
计算分子量10 kDa
观测分子量 11 kDa
GenBank蛋白编号NM_013650.2
基因名称 S100a8
Gene ID (NCBI) 20201
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP27005
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

实验方案

Product Specific Protocols
WB protocol for S100A8 antibody 32681-1-APDownload protocol
IF protocol for S100A8 antibody 32681-1-APDownload protocol
IP protocol for S100A8 antibody 32681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols