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验证数据展示

  • WB analysis using 10210-2-AP (same clone as 10210-2-PBS)

    WB analysis using 10210-2-AP (same clone as 10210-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10210-2-AP (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10210-2-AP (same clone as 10210-2-PBS)

    IP experiment of HeLa using 10210-2-AP (same clone as 10210-2-PBS)

    IP result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

    IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RUVBL1 antibody included
  • IF Staining of A431 using 10210-2-AP (same clone as 10210-2-PBS)

    IF Staining of A431 using 10210-2-AP (same clone as 10210-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RUVBL1 antibody (10210-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RUVBL1 Polyclonal antibody, PBS Only

RUVBL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10210-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RUVBL1 Ready-To-Use IHC Kit. RUVBL1 primary antibody included.

产品信息

10210-2-PBS targets RUVBL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0281

Product name: Recombinant human RUVBL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-214 aa of BC002993

Sequence: MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RuvB-like 1 (E. coli)
别名
计算分子量 456 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002993
基因名称 RUVBL1
Gene ID (NCBI) 8607
RRIDAB_2184405
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y265
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.

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