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验证数据展示

  • WB analysis of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    WB analysis of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

    IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

    IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    IP experiment of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    IP result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    IF Staining of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

RPL7 Polyclonal antibody, PBS Only

RPL7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14583-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14583-1-PBS targets RPL7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6104

Product name: Recombinant human RPL7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC006095

Sequence: MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L7
别名
计算分子量 29 kDa
观测分子量 29-30 kDa
GenBank蛋白编号BC006095
基因名称 RPL7
Gene ID (NCBI) 6129
RRIDAB_2254049
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18124
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

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