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验证数据展示

  • WB analysis of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    WB analysis of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    IP experiment of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    IP result of anti-RNF8 (IP:14112-1-AP, 4ug; Detection:14112-1-AP 1:600) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 14112-1-AP (same clone as 14112-1-PBS)

    IHC staining of human testis using 14112-1-AP (same clone as 14112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • WB analysis using 14112-1-AP (same clone as 14112-1-PBS)

    WB analysis using 14112-1-AP (same clone as 14112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    IF Staining of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF8 antibody (14112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RNF8 Polyclonal antibody, PBS Only

RNF8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14112-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14112-1-PBS targets RNF8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5292

Product name: Recombinant human RNF8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 130-485 aa of BC007517

Sequence: ETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVSCESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSSASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLCAEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWALMEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAHSFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERKAKRLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 8
别名
计算分子量 485 aa, 56 kDa
观测分子量 56-60 kDa
GenBank蛋白编号BC007517
基因名称 RNF8
Gene ID (NCBI) 9025
RRIDAB_2878016
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76064
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.

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