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验证数据展示

  • WB analysis using 20437-1-AP (same clone as 20437-1-PBS)

    WB analysis using 20437-1-AP (same clone as 20437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • IF Staining of SW480 using 20437-1-AP (same clone as 20437-1-PBS)

    IF Staining of SW480 using 20437-1-AP (same clone as 20437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RNF6 Polyclonal antibody, PBS Only

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20437-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RNF6 Ready-To-Use IHC Kit. RNF6 primary antibody included.

产品信息

20437-1-PBS targets RNF6 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14209

Product name: Recombinant human RNF6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC034688

Sequence: MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSEELQQRLDGVKEQLASQPDLRDGTNYRDSEVPRESSHEDSLLEWLNTFRRTGNATRSGQNGNQTWRAVSRTNPNNGEFRFSLEIHVNHENRGFEIHGEDYTDIPLSDSNRDHTANRQQRSTSPVARRTRSQTSVNFNGSSSNIPRTRLASRGQNPAEGSFSTLGRLRNGIGGAAGIPRANASRTNFSSHTNQSGGSELRQREGQRFGAAHVWENGARSNVTVRNTNQRLEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein (C3H2C3 type) 6
别名 E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2
计算分子量 685 aa, 78 kDa
观测分子量 78 kDa
GenBank蛋白编号BC034688
基因名称 RNF6
Gene ID (NCBI) 6049
RRIDAB_10666853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y252
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].

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