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验证数据展示

  • WB analysis of SH-SY5Y using 24618-1-AP (same clone as 24618-1-PBS)

    WB analysis of SH-SY5Y using 24618-1-AP (same clone as 24618-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

  • WB analysis using 24618-1-AP (same clone as 24618-1-PBS)

    WB analysis using 24618-1-AP (same clone as 24618-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 24618-1-AP (same clone as 24618-1-PBS)

    IF Staining of HaCaT using 24618-1-AP (same clone as 24618-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using RNF167 antibody (24618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RNF167 Polyclonal antibody, PBS Only

RNF167 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24618-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RNF167 Ready-To-Use IHC Kit. RNF167 primary antibody included.

产品信息

24618-1-PBS targets RNF167 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18383

Product name: Recombinant human RNF167 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-200 aa of BC010139

Sequence: IRATSDHNASMDFADLPALFGATLSQEGLQGFLVEAHPDNACSPIAPPPPAPVNGSVFIALLRRFDCNFDLKVLNAQKAGYGAAVVHNVNSNELLNMVWNSEEIQQQIWIPSVFIGERSSEYLRALFVYEKGARVLLVPDNTFPLGYYLIPFTGIVGLLVLAMGAVMIARCIQHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 167
别名 E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105
计算分子量 350 aa, 38 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC010139
基因名称 RNF167
Gene ID (NCBI) 26001
RRIDAB_2879642
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Y7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).

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