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验证数据展示

  • WB analysis using 85137-4-RR (same clone as 85137-4-PBS)

    WB analysis using 85137-4-RR (same clone as 85137-4-PBS)

    Untreated and PNGase F-treated, PNGase F inhibitor-treated HT-29 cells were subjected to SDS PAGE followed by western blot with 85137-4-RR (RNF128 antibody) at a dilution of 1:1000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85137-4-PBS in a different storage buffer formulation.

  • WB analysis using 85137-4-RR (same clone as 85137-4-PBS)

    WB analysis using 85137-4-RR (same clone as 85137-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85137-4-RR (RNF128 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85137-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85137-4-RR

    Affinity and Kinetic Characterization of 85137-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85137-4-RR against Human RNF128 were performed. The affinity constant is 3.82 nM.

RNF128 Recombinant monoclonal antibody, PBS Only

RNF128 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85137-4-PBS

产品说明书

CloneNo. 242759G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

E3 ubiquitin-protein ligase RNF128, EC:2.3.2.27, GRAIL, RING finger protein 128, RING-type E3 ubiquitin transferase RNF128

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85137-4-PBS targets RNF128 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22834

Product name: Recombinant human RNF128 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 333-428 aa of BC063404

Sequence: DGSVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 128
别名 E3 ubiquitin-protein ligase RNF128, EC:2.3.2.27, GRAIL, RING finger protein 128, RING-type E3 ubiquitin transferase RNF128
计算分子量 428 aa, 47 kDa
观测分子量70 kDa
GenBank蛋白编号BC063404
基因名称 RNF128
Gene ID (NCBI) 79589
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TEB7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF128 (Ring Finger Protein 128), also known as GRAIL (Gene Related to Anergy in Lymphocytes), is an E3 ubiquitin ligase that plays key roles in immune regulation, tumor biology, and antiviral responses. Initially identified in T cells, it is highly expressed in anergic CD4+ T cells and contributes to the induction and maintenance of T-cell anergy by catalyzing the ubiquitination and degradation of critical T-cell signaling molecules such as CD40L, CD80, and Rho-family GTPases.

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