验证数据展示
经过测试的应用
| Positive WB detected in | COLO 205 cells, HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85137-4-RR targets RNF128 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag22834 Product name: Recombinant human RNF128 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 333-428 aa of BC063404 Sequence: DGSVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ring finger protein 128 |
| 别名 | E3 ubiquitin-protein ligase RNF128, EC:2.3.2.27, GRAIL, RING finger protein 128, RING-type E3 ubiquitin transferase RNF128 |
| 计算分子量 | 428 aa, 47 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC063404 |
| 基因名称 | RNF128 |
| Gene ID (NCBI) | 79589 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8TEB7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF128 (Ring Finger Protein 128), also known as GRAIL (Gene Related to Anergy in Lymphocytes), is an E3 ubiquitin ligase that plays key roles in immune regulation, tumor biology, and antiviral responses. Initially identified in T cells, it is highly expressed in anergic CD4+ T cells and contributes to the induction and maintenance of T-cell anergy by catalyzing the ubiquitination and degradation of critical T-cell signaling molecules such as CD40L, CD80, and Rho-family GTPases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF128 antibody 85137-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
