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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12473-1-AP (same clone as 12473-1-PBS)

    WB analysis using 12473-1-AP (same clone as 12473-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12473-1-AP (RIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP experiment of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP experiment of rat brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    IHC staining of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 12473-1-AP (same clone as 12473-1-PBS)

    IHC staining of mouse pancreas using 12473-1-AP (same clone as 12473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

RIT2 Polyclonal antibody, PBS Only

RIT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12473-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12473-1-PBS targets RIT2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RIT2 fusion protein Ag3160 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-like without CAAX 2
别名
计算分子量 217 aa, 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC018060
基因名称 RIT2
Gene ID (NCBI) 6014
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99578
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).