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验证数据展示

  • WB analysis of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    WB analysis of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    IF Staining of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293T using 18825-1-AP (same clone as 18825-1-PBS)

    WB analysis of HEK-293T using 18825-1-AP (same clone as 18825-1-PBS)

    WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    IP experiment of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

    IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

    IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RBP2 Polyclonal antibody, PBS Only

RBP2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 18825-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IF-P, IP, Indirect ELISA

别名

KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18825-1-PBS targets RBP2 in WB, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysine (K)-specific demethylase 5A
别名 KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A
计算分子量 192 kDa
观测分子量 192 kDa
GenBank蛋白编号NM_005056
基因名称 KDM5A
Gene ID (NCBI) 5927
RRIDAB_10638436
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29375
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.