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验证数据展示

  • IHC staining of rat heart using 31177-1-AP (same clone as 31177-1-PBS)

    IHC staining of rat heart using 31177-1-AP (same clone as 31177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31177-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 31177-1-AP (same clone as 31177-1-PBS)

    IHC staining of mouse heart using 31177-1-AP (same clone as 31177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31177-1-PBS in a different storage buffer formulation.

RBM20 Polyclonal antibody, PBS Only

RBM20 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 31177-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, Indirect ELISA

别名

RNA binding motif protein 20, RNA binding protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31177-1-PBS targets RBM20 in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34322

Product name: Recombinant human RBM20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 667-840 aa of NM_001134363

Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 20
别名 RNA binding motif protein 20, RNA binding protein
计算分子量134 kDa
GenBank蛋白编号NM_001134363
基因名称 RBM20
Gene ID (NCBI) 282996
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5T481
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM20 (RNA binding motif protein 20) is a striated muscle-specific splicing factor that regulates alternative splicing of many cardiac genes encoding sarcomere and calcium-regulatory proteins (PMID: 36417486). RBM20 has been identified as a crucial RNA-binding protein that controls the splicing of TTN (PMID: 30547036). RBM20 is expressed in all striated muscles but is highest in cardiac tissue (PMID: 34575212).

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