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验证数据展示

  • WB analysis using 31122-1-AP

    WB analysis using 31122-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31122-1-AP (RAGE antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 31122-1-AP

    IHC staining of mouse lung using 31122-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31122-1-AP (RAGE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAGE Polyclonal antibody

RAGE Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31122-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31122-1-AP targets RAGE in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 RAGE fusion protein Eg0873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1
计算分子量43 kDa
观测分子量42-55 kDa
GenBank蛋白编号NM_007425.3
基因名称 Ager
Gene ID (NCBI) 11596
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ62151-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).

实验方案

Product Specific Protocols
WB protocol for RAGE antibody 31122-1-APDownload protocol
IHC protocol for RAGE antibody 31122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols