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验证数据展示

  • IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

    IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

    IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

    WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

RABIF Polyclonal antibody, PBS Only

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29113-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29113-1-PBS targets RABIF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30761

Product name: Recombinant human RABIF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of BC018488

Sequence: MEPAEQPSELVSAEGRNRKAVLCQRCGSRVLQPGTALFSRRQLFLPSMRKKPALSDGSNPDGDLLQEHWLVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB interacting factor
别名
计算分子量 123 aa, 14 kDa
观测分子量14 kDa
GenBank蛋白编号BC018488
基因名称 RABIF
Gene ID (NCBI) 5877
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47224
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).

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