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验证数据展示

  • IHC staining of mouse brain using 29113-1-AP

    IHC staining of mouse brain using 29113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29113-1-AP

    WB analysis using 29113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 29113-1-AP

    IF Staining of MCF-7 using 29113-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

RABIF Polyclonal antibody

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29113-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

MSS4, RAB interacting factor, RABIF, RASGFR3, RASGRF3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29113-1-AP targets RABIF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag30761

Product name: Recombinant human RABIF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of BC018488

Sequence: MEPAEQPSELVSAEGRNRKAVLCQRCGSRVLQPGTALFSRRQLFLPSMRKKPALSDGSNPDGDLLQEHWLVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB interacting factor
别名 MSS4, RAB interacting factor, RABIF, RASGFR3, RASGRF3
计算分子量 123 aa, 14 kDa
观测分子量14 kDa
GenBank蛋白编号BC018488
基因名称 RABIF
Gene ID (NCBI) 5877
RRIDAB_3669674
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).

实验方案

Product Specific Protocols
IF protocol for RABIF antibody 29113-1-APDownload protocol
IHC protocol for RABIF antibody 29113-1-APDownload protocol
WB protocol for RABIF antibody 29113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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