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验证数据展示

  • WB analysis of HeLa using 87525-1-RR (same clone as 87525-1-PBS)

    WB analysis of HeLa using 87525-1-RR (same clone as 87525-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87525-1-RR (Phospho-PFKFB2 (Ser483) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87525-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 87525-1-RR (same clone as 87525-1-PBS)

    WB analysis of MCF-7 using 87525-1-RR (same clone as 87525-1-PBS)

    Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87525-1-RR (Phospho-PFKFB2 (Ser483) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87525-1-PBS in a different storage buffer formulation.

Phospho-PFKFB2 (Ser483) Recombinant monoclonal antibody, PBS Only

Phospho-PFKFB2 (Ser483) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87525-1-PBS

产品说明书

CloneNo. 252874F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

PFKFB2, 6PF-2-K/Fru-2,6-P2ase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87525-1-PBS targets Phospho-PFKFB2 (Ser483) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
别名 PFKFB2, 6PF-2-K/Fru-2,6-P2ase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
计算分子量58 kDa
观测分子量55 kDa
GenBank蛋白编号NM_006212
基因名称 PFKFB2
Gene ID (NCBI) 5208
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60825
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phospho-PFKFB2 (Ser483) is the active-site-switching modification of the heart-type bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2). Phosphorylation at serine 483, located in the C-terminal regulatory segment, is catalyzed mainly by Akt downstream of PI3K and is essential for maximal 2-kinase activity: it increases the synthesis of fructose-2,6-bisphosphate (F2,6BP), the most potent allosteric activator of 6-phosphofructo-1-kinase (PFK-1), thereby accelerating glycolytic flux and ATP production. (PMID: 34282152)

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