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CoraLite®555-conjugated Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3N6

Cat No : CL555-80340

Print datasheet

Synonyms

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

验证数据展示

  • FC experiment of HeLa using CL555-80340

    FC experiment of HeLa using CL555-80340

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL555-80340, Clone:3N6) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

  • FC experiment of HeLa using CL555-80340

    FC experiment of HeLa using CL555-80340

    1X10^6 HeLa cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL555-80340, Clone:3N6) (red), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

经过测试的应用

Positive FC detected inCalyculin A treated HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 80340-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-80340 targets Phospho-Hamartin/TSC1 (Ser511) in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tuberous sclerosis 1
Synonyms Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein
Calculated Molecular Weight 130 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberNM_000368
Gene Symbol TSC1
Gene ID (NCBI) 7248
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92574
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).