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验证数据展示

  • IP experiment of HepG2 using 55429-1-AP

    IP experiment of HepG2 using 55429-1-AP

    IP result of anti-PYGL (IP:55429-1-AP, 4ug; Detection:55429-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 55429-1-AP

    IF Staining of HepG2 using 55429-1-AP

    Immunofluorescent analysis of HepG2 cells using 55429-1-AP (PYGL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PYGL Polyclonal antibody

PYGL Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 55429-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

GSD6, phosphorylase, glycogen, liver, PYGL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, mouse liver
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55429-1-AP targets PYGL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylase, glycogen, liver
别名 GSD6, phosphorylase, glycogen, liver, PYGL
计算分子量 97 kDa
观测分子量 97-105 kDa
GenBank蛋白编号NM_002863
基因名称 PYGL
Gene ID (NCBI) 5836
RRIDAB_11182599
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06737
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.

实验方案

Product Specific Protocols
WB protocol for PYGL antibody 55429-1-APDownload protocol
IHC protocol for PYGL antibody 55429-1-APDownload protocol
IF protocol for PYGL antibody 55429-1-APDownload protocol
IP protocol for PYGL antibody 55429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols