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PYGL Polyclonal antibody

PYGL Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 55429-1-AP

Print datasheet

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL

验证数据展示

  • WB analysis of HepG2 using 55429-1-AP

    WB analysis of HepG2 using 55429-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55429-1-AP (PYGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 55429-1-AP

    IP experiment of HepG2 using 55429-1-AP

    IP result of anti-PYGL (IP:55429-1-AP, 4ug; Detection:55429-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 55429-1-AP

    IF Staining of HepG2 using 55429-1-AP

    Immunofluorescent analysis of HepG2 cells using 55429-1-AP (PYGL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, mouse liver
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55429-1-AP targets PYGL in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylase, glycogen, liver
Synonyms GSD6, phosphorylase, glycogen, liver, PYGL
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberNM_002863
Gene Symbol PYGL
Gene ID (NCBI) 5836
RRIDAB_11182599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06737
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.

实验方案

Product Specific Protocols
WB protocol for PYGL antibody 55429-1-APDownload protocol
IHC protocol for PYGL antibody 55429-1-APDownload protocol
IF protocol for PYGL antibody 55429-1-APDownload protocol
IP protocol for PYGL antibody 55429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols