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验证数据展示

  • WB analysis of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    WB analysis of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    IP experiment of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    IP result of anti-PSME2 (IP:12937-2-AP, 4ug; Detection:12937-2-AP 1:500) with MCF-7 cells lysate 2000ug. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

    IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSME2 antibody included
  • IF Staining of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    IF Staining of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME2 antibody (12937-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

PSME2 Polyclonal antibody, PBS Only

PSME2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12937-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

REG-beta, REGbeta, REG beta, PA28beta, PA28B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSME2 Ready-To-Use IHC Kit. PSME2 primary antibody included.

产品信息

12937-2-PBS targets PSME2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSME2 fusion protein Ag3986 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
别名 REG-beta, REGbeta, REG beta, PA28beta, PA28B
计算分子量 239 aa, 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC019885
基因名称 PSME2
Gene ID (NCBI) 5721
RRIDAB_2300265
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UL46
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.