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PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for WB, IHC, ELISA, IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG


human, mouse, rat


WB, IHC, ELISA, IF/ICC, FC (Intra) and More (1)



Cat No : 14899-1-AP

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Multiubiquitin chain-binding protein, MCB1, ASF, AF, 26S proteasome non-ATPase regulatory subunit 4


Positive WB detected inJurkat cells, rat spleen tissue, K-562 cells, HeLa cells, NIH/3T3 cells, RAW264.7 cells, Raji cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells


Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


14899-1-AP targets PSMD4 in WB, IHC, ELISA, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA, IF/ICC, FC (Intra) Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PSMD4 fusion protein Ag6691 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Synonyms Multiubiquitin chain-binding protein, MCB1, ASF, AF, 26S proteasome non-ATPase regulatory subunit 4
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002365
Gene Symbol PSMD4
Gene ID (NCBI) 5710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.


Product Specific Protocols
WB protocol for PSMD4 antibody 14899-1-APDownload protocol
IHC protocol for PSMD4 antibody 14899-1-APDownload protocol
IF protocol for PSMD4 antibody 14899-1-APDownload protocol
FC protocol for PSMD4 antibody 14899-1-APDownload protocol
Standard Protocols
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Nat Neurosci

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Authors - Rossella Avagliano Trezza

J Cell Biol

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

Authors - Christoph Gerhardt

Cancer Res

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Authors - Xiuxia Liu


The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A

J Cell Sci

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Authors - Zheng Wang

Mol Cell Proteomics

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Authors - Tatjana Goebel