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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

    WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

  • WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

    WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

    IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB7 antibody included
  • IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

    IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB7 antibody included

PSMB7 Polyclonal antibody, PBS Only

PSMB7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30283-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMB7 Ready-To-Use IHC Kit. PSMB7 primary antibody included.

产品信息

30283-1-PBS targets PSMB7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32201

Product name: Recombinant human PSMB7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 91-277 aa of BC000509

Sequence: TAADTDMTTQLISSNLELHSLSTGRLPRVVTANRMLKQMLFRYQGYIGAALVLGGVDVTGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAVFEDKFRPDMEEEEAKNLVSEAIAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKGTRLGRYRCEKGTTAVLTEKITPLEIEVLEETVQTMDTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 7
别名
计算分子量29.9 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC000509
基因名称 PSMB7
Gene ID (NCBI) 5695
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99436
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)