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验证数据展示

  • WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IP result of anti-PRKCSH (IP:12148-1-AP, 4ug; Detection:12148-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCSH antibody included
  • IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCSH antibody included
  • IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12148-1-AP (PRKCSH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

    IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCSH antibody included
  • Featured Product
  • KD/KO验证

PRKCSH Polyclonal antibody, PBS Only

PRKCSH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12148-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKCSH Ready-To-Use IHC Kit. PRKCSH primary antibody included.

产品信息

12148-1-PBS targets PRKCSH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2796

Product name: Recombinant human PRKCSH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-506 aa of BC013586

Sequence: TVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPTPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C substrate 80K-H
别名
计算分子量 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC013586
基因名称 PRKCSH
Gene ID (NCBI) 5589
RRIDAB_2253107
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14314
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.

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