- Featured Product
- KD/KO Validated
PRDX4 Polyclonal antibody
PRDX4 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HepG2 cells, HEK-293 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10703-1-AP targets PRDX4 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | PRDX4 fusion protein Ag1177 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | peroxiredoxin 4 |
| Synonyms | Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC007107 |
| Gene Symbol | PRDX4 |
| Gene ID (NCBI) | 10549 |
| RRID | AB_2168493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13162 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRDX4 antibody 10703-1-AP | Download protocol |
| IHC protocol for PRDX4 antibody 10703-1-AP | Download protocol |
| IF protocol for PRDX4 antibody 10703-1-AP | Download protocol |
| IP protocol for PRDX4 antibody 10703-1-AP | Download protocol |
| FC protocol for PRDX4 antibody 10703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma | ||
Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. | ||
Antioxid Redox Signal Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I. | ||
Bioorg Chem Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents. | ||
FEBS J A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems. |
