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验证数据展示

  • WB analysis of human brain using 14573-1-AP (same clone as 14573-1-PBS)

    WB analysis of human brain using 14573-1-AP (same clone as 14573-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

    IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

    IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PPM1K Polyclonal antibody, PBS Only

PPM1K Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14573-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14573-1-PBS targets PPM1K in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5660

Product name: Recombinant human PPM1K protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-372 aa of BC037552

Sequence: MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1K (PP2C domain containing)
别名
计算分子量 41 kDa
观测分子量30-40 kDa
GenBank蛋白编号BC037552
基因名称 PPM1K
Gene ID (NCBI) 152926
RRIDAB_2252953
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3J5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).

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