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验证数据展示

  • WB analysis of HeLa using 18001-1-AP (same clone as 18001-1-PBS)

    WB analysis of HeLa using 18001-1-AP (same clone as 18001-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

    IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

    IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • WB analysis using 18001-1-AP (same clone as 18001-1-PBS)

    WB analysis using 18001-1-AP (same clone as 18001-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

PPCS Polyclonal antibody, PBS Only

PPCS Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18001-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

COAB, EC:6.3.2.51, Phosphopantothenate--cysteine ligase, PPC synthetase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18001-1-PBS targets PPCS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12491

Product name: Recombinant human PPCS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-311 aa of BC106064

Sequence: MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFIGDRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphopantothenoylcysteine synthetase
别名 COAB, EC:6.3.2.51, Phosphopantothenate--cysteine ligase, PPC synthetase
计算分子量 311 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC106064
基因名称 PPCS
Gene ID (NCBI) 79717
RRIDAB_10548725
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAB8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

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