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验证数据展示

  • IF Staining of hTERT-RPE1 using 28750-1-AP (same clone as 28750-1-PBS)

    IF Staining of hTERT-RPE1 using 28750-1-AP (same clone as 28750-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

    IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLK4 antibody included
  • Featured Product
  • KD/KO验证

PLK4 Polyclonal antibody, PBS Only

PLK4 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 28750-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PLK4 Ready-To-Use IHC Kit. PLK4 primary antibody included.

产品信息

28750-1-PBS targets PLK4 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30482

Product name: Recombinant human PLK4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 235-426 aa of BC036023

Sequence: SIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polo-like kinase 4 (Drosophila)
别名
计算分子量 970 aa, 109 kDa
GenBank蛋白编号BC036023
基因名称 PLK4
Gene ID (NCBI) 10733
RRIDAB_2881206
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00444
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

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