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验证数据展示

  • WB analysis using 85743-2-RR (same clone as 85743-2-PBS)

    WB analysis using 85743-2-RR (same clone as 85743-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85743-2-RR (PELO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85743-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02111-2

    Cytometric bead array standard curve of MP02111-2

    Cytometric bead array standard curve of MP02111-2, PELO Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85743-3-PBS. Detection antibody: 85743-2-PBS. Standard: Ag0873. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP02111-1

    Cytometric bead array standard curve of MP02111-1

    Cytometric bead array standard curve of MP02111-1, PELO Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85743-1-PBS. Detection antibody: 85743-2-PBS. Standard: Ag0873. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 85743-2-RR

    Affinity and Kinetic Characterization of 85743-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85743-2-RR against Human PELO were performed. The affinity constant is 0.152 nM.

PELO Recombinant antibody, PBS Only (Detector)

PELO Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 85743-2-PBS

产品说明书

CloneNo. 243137A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85743-2-PBS targets PELO as part of a matched antibody pair:

MP02111-1: 85743-1-PBS capture and 85743-2-PBS detection (validated in Cytometric bead array)

MP02111-2: 85743-3-PBS capture and 85743-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PELO fusion protein Ag0873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pelota homolog (Drosophila)
别名 CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog
计算分子量 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC005889
基因名称 PELO
Gene ID (NCBI) 53918
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRX2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].