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验证数据展示

  • WB analysis using 68249-1-Ig

    WB analysis using 68249-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68249-1-Ig

    WB analysis using 68249-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 68249-1-Ig

    IF Staining of HEK-293 using 68249-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDCD2L antibody (68249-1-Ig, Clone: 2G12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PDCD2L Monoclonal antibody

PDCD2L Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68249-1-Ig

产品说明书

CloneNo. 2G12C4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

PDCD2L, programmed cell death 2 like

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HCT 116 cells, HeLa cells, K-562 cells, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68249-1-Ig targets PDCD2L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PDCD2L fusion protein Ag30807 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 2-like
别名 PDCD2L, programmed cell death 2 like
计算分子量 358 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC006146
基因名称 PDCD2L
Gene ID (NCBI) 84306
RRIDAB_3085049
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRP1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDCD2L was aberrantly expressed in multiple types of human cancers, and associated with clinical stage and molecular subtype and PDCD2L could be served as a biomarker of CRC (PMID: 35216602).

实验方案

Product Specific Protocols
WB protocol for PDCD2L antibody 68249-1-IgDownload protocol
IF protocol for PDCD2L antibody 68249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols