验证数据展示
经过测试的应用
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CPY5-FcA98166-4 targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
DNA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD274 antigen |
| 别名 | Cd274, PD-L1, B7 H1, PD L1, Pdcd1l1 |
| 计算分子量 | 33 kDa |
| GenBank蛋白编号 | NM_021893.3 |
| 基因名称 | PD-L1 |
| Gene ID (NCBI) | 60533 |
| 偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 690nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9EP73 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1) is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cells, and plays an important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFN gamma, in an IL-2-dependent and a PD-1-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PerCP-Cyanine5.5 PD-L1/CD274 antibody CPY5-FcA98166-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
