PCNA Monoclonal antibody
PCNA Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG
Reactivity
human, mouse
Applications
WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | 293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
65034-1-Ig targets PCNA in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB,ELISA |
Tested Reactivity | human, mouse |
Immunogen | Recombinant Protein 种属同源性预测 |
Host / Isotype | Mouse / IgG |
Class | Monoclonal |
Type | Antibody |
Full Name | proliferating cell nuclear antigen |
Synonyms | Cyclin, PCNA |
Calculated Molecular Weight | 29 kDa/31 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC000491 |
Gene Symbol | PCNA |
Gene ID (NCBI) | 5111 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P12004 |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at 2-8°C. |
背景介绍
PCNA, also named as Cyclin, belongs to the PCNA family. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. The PCNA protein has been highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. This antibody is a mouse monoclonal antibody raised against recombinant protein of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).